Coding

Part:BBa_K1593998

Designed by: Yuting Liu   Group: iGEM15_USTC   (2015-09-03)

overexpress cheZ

this part is uesd for coding cheZ, a chemotactic protein. When cheZ is overexpressed, the mobility of bacteria will be improved.


Chemotaxis

When presence of chemicals, cheA is free from cheW interaction, and cheZ will efficiently remove phosphate group from cheY.

4903.jpg

Stracture

PDB code:1KMI

4901.jpg


Growth characteristics

We detected OD600(nm) to identify the effect of cheZ expression on E. coli through time.

4902.jpg

The exogenous protein expression affects the growth of bacteria, which was indicated by slight slower grow-up in E. coli containing cheZ plasmid. In total, cheZ doesn't have obvious negative impact on bacteria grown.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 373
    Illegal NgoMIV site found at 508
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None